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Antibodies sure to the blocking peptide not bind for the epitope around the concentrate on protein. By comparing the staining in the blocked antibody vs . the antibody on your own, you can see which staining is particular.

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This information outlines additional feasible optimization of cellular and immunoassay parameters to ensure the absolute best effects are...

This guidebook outlines further achievable optimization of mobile and immunoassay parameters to be sure the absolute best effects are...

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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Clicking the pictures or backlinks will redirect you to definitely a website hosted by BenchSci that gives third-occasion scientific material. Neither the content nor the BenchSci technological know-how and processes for collection happen here to be evaluated by us; we have been providing them as-is and without having guarantee of any form, such as for use or application on the Thermo Fisher Scientific products and solutions introduced.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...

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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide usually has the epitope acknowledged via the antibody.

Goal Information and facts Cyclin E belongs towards the remarkably conserved cyclin loved ones, whose customers are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation patterns which contribute for the temporal coordination of every mitotic function. Cyclin E varieties a posh with and capabilities for a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates on the G1-S stage boundary and it is degraded as cells progress via S phase.

Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two extra splice variants ended up noted but in-depth nucleotide sequence details is not really however readily available.

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